Anicura is a natural treatment not medicine, so it is safe to use on pregnant dogs/cats. However, we always recommend you do a patch test to ensure your pet is not allergic to any of the natural ingredients. The best way to do a patch test is to apply a small amount in an area where your pet is less likely to lick it off such as the back of the neck. Apply the product as close to the skin as possible and wait for 24 hours before using it everywhere else. We like being extra safe with pregnant pets!